User Guide.... HERE

The kit is a broad-spectrum kit for the purification of RNA from cultured cells, animal tissues, plant tissues (young and tender stems, leaves) by column method. The protocol provides a simple method to achieve the rapid isolation of highly purified RNA and the entire procedure can be accomplished within 30 minutes after the tissues or cells being lysed and homogenized.The high purity RNA is extracted by gDNA Eraser Spin Column (to remove genomic DNA) and RNA Spin Column (to bind RNA).

The procedure avoids the phenol and chloroform extraction.. The purified RNA contains no protein and no genomic DNA contamination. The high-quality RNA can be used in Northern blotting, dot blotting, mRNA purification, in vitro translation, RNase protection assays, RT-PCR, Real Time RT-PCR, cDNA library construction and other molecular experiments.

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