• 2x Universal Ligation Mix is a ready-to-use 2x master mix containing T4 DNA Ligase and reaction buffer. The contained T4 DNA Ligase catalyzes the binding of phosphodiester bonds between the 5'-P and 3'-OH ends of sticky-end or blunt-ended double-stranded DNA or RNA. At the same time, it can repair single-stranded nicks in double-stranded DNA, RNA, and DNA/RNA hybrid strands.
• The optimized premixed reaction buffer makes the reaction more efficient and easier to operate. The reaction system can transform a variety of chemically competent cells after 5 min at 25°C.
  

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