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Trypsin, abbreviated as trypsin, is a serine hydrolase that cuts off the carboxyl termini of lysine and arginine residues in polypeptide chains
This product is a 0.25% trypsin digestion solution, containing 0.25% trypsin, dissolved in Hank's buffer, containing 0.9 mM EDTA, no phenol red indicator, sterilized by 0.22μm filtration.
- In the process of tissue cell extraction, culture, and in vitro cell culture, trypsin digestion solution is used to hydrolyze intercellular proteins, so that the tissue or cell can be dispersed into a single cell. The digestion ability of trypsin was the strongest at 37℃ pH 8.0. The common working concentration of trypsin is 0.25%. EDTA can chelate calcium and magnesium ions. The use of EDTA in trypsin solution can accelerate the hydrolysis of intercellular junction proteins and enhance digestion. Phenol red is a pH indicator. The solution is purplish-red when it is more basic, orange-red when it is more acidic, and pink when it is near neutral.
Trypsin EDTA (0.25%)
- Product Code: Bi2M-EDTA-25
- Availability: In Stock